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目的:观察具有免疫抑制作用的药物黄柏及其3种主要成分:小檗碱、药根碱与巴马汀对正常小鼠脾细胞膜流动性的影响。方法:以1,6-二苯基,1,3,5-己三烯(DPH)作为荧光探针,利用荧光偏振法测定脾细胞膜脂质区的流动性。结果:在无ConA刺激时,黄柏的水提物可提高小鼠脾细胞膜的流动性,6.25%的浓度与对照组相比有极显著性差异;小檗碱与药根碱可降低脾细胞膜的流动性,与对照组相比均有显著性差异;巴马汀可提高脾细胞膜的流动性,与对照组相比有极显著性差异。经ConA刺激后,黄柏的水提物、小檗碱、药根碱及巴马汀均可降低脾细胞膜流动性,与对照组相比有显著性差异。结论:上述结果提示这些药物的免疫抑制作用有可能是通过降低淋巴细胞膜的流动性来实现的。
OBJECTIVE: To observe the effect of phellodendron chinense and its three main components: berberine, jatrorrhizine and palmatine on the splenocyte membrane fluidity of normal mice. Methods: 1,6-diphenyl, 1,3,5-hexatriene (DPH) was used as a fluorescent probe, and the mobility of lipids in splenocyte membrane was determined by fluorescence polarization method. Results: In the absence of Con A stimulation, the aqueous extract of Phellodendron can increase the membrane fluidity of spleen cell membrane in mice. The concentration of 6.25% was significantly different from that of the control group. Berberine and jatrorrhizine can reduce the splenic cell membrane. The fluidity was significantly different from that of the control group; palmatine could improve the fluidity of the spleen cell membrane, and there was a very significant difference compared with the control group. After ConA stimulation, the water extract of Phellodendron amurense, berberine, jatrorrhizine and palmatine all reduced the spleen cell membrane fluidity, and there was a significant difference compared with the control group. Conclusion: These results suggest that the immunosuppressive effects of these drugs may be achieved by reducing the fluidity of the lymphocyte membrane.