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目的:分析anti-TCRαβ mAb、anti-TCRαβ mAb+anti-CD28mAb诱导小鼠胸腺淋巴细胞不同亚群的凋亡及凋亡程度,分析CD28协同刺激分子对TCR受体介导的胸腺细胞亚群凋亡 的影响。方法:新鲜分离胸腺细胞,加入anti-TCRαβ mAb、anti-TCRαβ mAb+anti-CD28 mAb等培养20 h,进行多重染色,流式细胞仪分析。结果:与胸腺细胞自发凋亡的结果相比较:①双信号刺激可明显增加胸腺细胞凋亡的数目,尤其是CD4+ CD8+胸腺细胞的凋亡数目。②凋亡的CD4+ CD8+亚群、CD4+ CD8-亚群细胞表面CD28的表达均增多。结论:CD28共刺激分子对TCR受体介导的胸腺细胞亚群凋亡的影响与细胞的成熟程度有关。CD28共刺激分子能明显增强不成熟皮质胸腺细胞的凋亡。
OBJECTIVE: To analyze the apoptosis and apoptosis of thymus lymphocyte subsets induced by anti-TCRαβ mAb and anti-TCRαβ mAb + anti-CD28mAb, and to analyze the effect of CD28 costimulatory molecules on TCR receptor-mediated thymocyte subpopulation The impact of death. Methods: Thymocytes were freshly isolated and cultured with anti-TCRαβ mAb and anti-TCRαβ mAb + anti-CD28 mAb for 20 h. The cells were analyzed by flow cytometry. Results: Compared with the results of spontaneous apoptosis of thymocytes, ① The number of apoptotic thymocytes significantly increased, especially the number of CD4 + CD8 + thymocytes. ② The expression of CD28 on the surface of CD4 + CD8 + subsets and CD4 + CD8- subsets increased. Conclusion: The effect of CD28 costimulatory molecules on TCR receptor-mediated thymocyte subsets apoptosis is related to the degree of cell maturation. CD28 costimulatory molecules can significantly enhance the apoptosis of immature cortical thymocytes.