论文部分内容阅读
目的探讨福建省汉族人群三磷腺苷结合转运子A1基因(ABCA1)R219K单核苷酸多态性(SNP)和子痫前期(preeclampsia)及其血脂水平的关联性。方法研究对象621例,包括对照组316例,子痫前期病例组305例。采用聚合酶链反应一限制性片段长度多态性(PCR-RFLP)法鉴定ABCA1基因外显子区R219K单核苷酸多态性并测定血脂水平。结果 ABCA1基因R219K位点K等位基因频率和RK+KK基因型频率在子痫前期病例组明显降低,差异有高度统计学意义P<0.001)。子痫前期病例组内RK+KK基因型患者血清甘油三酯(TG)浓度低于RR基因型,而HDL-C浓度刚好相反,差异有高度统计学意义(P<0.001)。结论福建省汉族人群ABCA1基因R219K中,K等位基因可能是子痫前期的独立的保护因子,其机制可能是通过提高血液中HDL-C水平,降低TG水平,从而降低了子痫前期合并血脂代谢紊乱的风险性。
Objective To investigate the association between the ABCA1 R219K single nucleotide polymorphism (SNP) and preeclampsia and its serum lipids in Han nationality in Fujian province. Methods Six hundred and sixty-one subjects were included, including 316 patients in the control group and 305 patients in the preeclampsia group. The R219K SNP in exon of ABCA1 gene was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and lipid levels were determined. Results The allele frequencies of K allele and RK + KK at R219K locus in ABCA1 gene were significantly decreased in preeclampsia patients (P <0.001). Serum triglyceride (TG) concentrations were lower in RK + KK genotype patients than in RR genotypes in preeclampsia patients, but HDL-C concentrations were just the opposite, with a statistically significant difference (P <0.001). Conclusions The K allele of R219K in ABCA1 gene of Fujian Han population may be an independent protective factor in preeclampsia. The mechanism may be that it can reduce HDL-C in blood and decrease TG level, thereby reducing the risk of preeclampsia with lipid Risk of metabolic disorders.