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目的:研究柴胡皂苷a(SSa)对氯化锂-匹鲁卡品致难治性癫痫模型大鼠多药耐药蛋白P-糖蛋白(P-gp)表达的影响。方法:将制备成功的30只SD难治性癫痫大鼠随机分为5组,丙戊酸钠(VPA)组以VPA 200 mg.kg-1灌胃给药,SSa低、中、高剂量组分别ip SSa 0.54,1.09,2.18 mg.kg-1,空白对照组(A)和模型组(B)均等容积生理盐水ig,各组连续给药8周后用Western blotting检测各组大鼠海马、颞叶皮层P-gp的表达。结果:在大鼠海马、颞叶皮层,各组P-gp的表达,差异有统计学意义(P<0.001)。与空白对照组相比,其余各组P-gp表达水平明显增强(P<0.05);与模型组相比,VPA 200 mg.kg-1组表达水平增加(P<0.05),SSa 3个剂量组表达水平降低,以SSa高剂量组最明显(P<0.05)。结论:SSa低、中、高剂量均可降低大鼠颞叶皮层、海马区P-gp的表达,其效果成剂量依赖性,以高剂量组效果明显。
Objective: To study the effects of saikosaponin a (SSa) on the expression of P-glycoprotein (P-gp) in rats with intractable epilepsy induced by lithium chloride-pilocarpine. Methods: Thirty SD-refractory epilepsy rats were randomly divided into five groups. The VPA group was given intragastrically VPA 200 mg.kg-1, while the SSa low, middle and high dose group The rats in each group were administrated with ip SSa 0.54,1.09,2.18 mg.kg-1, the blank control group (A) and the model group (B) Expression of P-gp in temporal cortex. Results: The expression of P-gp in hippocampus and temporal cortex of rats was significantly different (P <0.001). Compared with the blank control group, the expression levels of P-gp in the other groups were significantly increased (P <0.05). Compared with the model group, the expression level of VPA 200 mg.kg-1 increased (P <0.05) The expression level in the group of SSa was the highest (P <0.05). CONCLUSION: Low, medium and high doses of SSa can reduce the expression of P-gp in the temporal cortex and hippocampus in rats in a dose-dependent manner.