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通过室内人工饲养与枣树林间定时定点观测相结合的方法,对皮暗斑螟的生殖生物学进行观测。傍晚(17—20时)羽化的成虫占总量的45.71%,交尾多于午夜后至凌晨前进行。在室内通过气相色谱与触角电位测定相结合的方法,测定皮暗斑螟雄蛾对来自雌蛾性腺的不同化合物的反应,通过应用全二维气相色谱飞行时间质谱分析技术,初步明确了皮暗斑螟性信息素组分为(Z9,E12)-十四碳-9,12-二烯-1-醇[(Z,E)-9,12-14∶OH]和(Z9)-十四碳-9-烯-1醇(Z9-14∶OH)。对二组分及其同分异构体的单体和混合物进行了林间引诱试验,初步结果表明(Z,E)-9,12-14∶OH的诱虫效果较好。5,50,500和1000μg不同剂量诱芯的田间引诱试验证明,500μg剂量诱芯诱虫量最多。
Reproductive biology of Culicidae was studied through a combination of indoor artificial feeding and regular observation of fixed point in jujube tree. In the evening (17-20 hours) emergence of adults accounted for 45.71% of the total, more than the midnight to midnight to early morning. In the laboratory, the reaction between male and female moth gonads was determined by the combination of gas chromatography and potentiometry. By using full-dimensional gas chromatography time-of-flight mass spectrometry, (Z9, E12) -tetradeca-9,12-dien-1-ol [(Z, E) -9,12-14: OH] and (Z9) -tetradecyl- Carbon-9-en-1 alcohol (Z9-14: OH). The results showed that (Z, E) -9,12-14:OH had good trapping effect. The field induction experiments with 5, 50, 500 and 1000 μg different doses of entrapment proved that the 500 μg dose induced the most attractant.