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采用cDNA消减杂交和体内选择技术,Garkartsev等分离了一个新的肿瘤抑制基因—ING1。该基因定位于人类染色体13p33-34,其cDNA长约2kb;编码一种分子量为33kD的核蛋白——p33~(ING1)。研究发现,p33~(ING1)的过表达可有效抑制细胞生长,促进无生长因子条件下的细胞凋亡。在抑制细胞生长过程中,p33~(ING1)与p53相互依赖,具有协同作用。对一些肿瘤细胞系的研究表明,在成纤维细胞瘤中ING1基因的3′端发生缺失,在乳腺癌细胞中p33~(ING1)表达减低。
Using cDNA subtractive hybridization and in vivo selection techniques, Garkartsev et al. isolated a novel tumor suppressor gene, ING1. The gene is located on human chromosome 13p33-34, and its cDNA is about 2kb in length. It encodes a 33kD nuclear protein called p33~(ING1). It was found that overexpression of p33~(ING1) can effectively inhibit cell growth and promote apoptosis in the absence of growth factors. In the inhibition of cell growth, p33 ING1 and p53 are interdependent and have a synergistic effect. Studies on some tumor cell lines have revealed that the 3’ end of the ING1 gene is deleted in fibroblastomas, and p33 ING1 expression is reduced in breast cancer cells.