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观察儿童T淋巴细胞低剂量辐射后DNA合成的刺激效应和对大剂量辐射的适应性反应。 8例儿童外周血T淋巴细胞培养后 ,予以不同的低剂量辐射 ,用14 C TdR (14 C 胸腺嘧啶核苷 )或3 H TdR (3 H 胸腺嘧啶核苷 )掺入法观察T淋巴细胞DNA合成 ,用掺入百分率和辐射损伤减轻百分数 (DRP )分别表达刺激效应和适应性反应。T淋巴细胞经 2、 5、 10cGy照射后14 C TdR掺入与 0cGy比较分别是 10 7 7%、 12 5 5 %、 133 8% ,明显增加了DNA合成。分别予以 0 5、 1 0、 1 5、 2 0、 3 0、 4 0cGy不同剂量辐射后 ,DRP分别是 2 0 8%、 33 2 %、 5 3 4 %、 4 9 0 %、 2 8 9%。 1 5cGy辐射后 6、 2 4、 4 8、 72h ,DRP分别是2 2 2 %、 5 3 4 %、 36 6 %、 11 1%。 1 5cGy辐射后 2 4h再予以 1、 3、 5、 7Gy不同大剂量辐射 ,DRP分别是 30 7%、 4 5 1%、2 6 4 %、 11 9%。儿童T淋巴细胞经适宜的低剂量辐射后可以明显增加DNA合成 ,1 5cGy辐射后 2 4h对 3Gy大剂量辐射适应性反应表达最为充分
To observe the stimulating effect of DNA synthesis and the adaptive response to high dose radiation after low dose radiation of T lymphocytes in children. T lymphocytes were isolated from 8 cases of peripheral blood T lymphocytes and then irradiated with different doses of low dose radiation. T lymphocyte DNA was detected by 14 C TdR (14 C thymidine) or 3 H TdR (3 H thymidine incorporation) Synthesis, with the percentage of incorporation and radiation damage reduction percentage (DRP), respectively, to stimulate the expression and adaptive response. Compared with 0cGy, the incorporation of 14 C TdR in T lymphocytes irradiated with 2, 5 and 10 cGy was 107.7%, 125.5% and 133.8% respectively, which significantly increased the DNA synthesis. After being given 0, 10, 15, 20, 30, 40 cGy doses respectively, the DRP was 28.0%, 33.2%, 53.4%, 49.0%, 299% . After 14 days of radiation, the DRPs were 22.2%, 53.4%, 36.6% and 11.1% respectively at 6, 24, 48 and 72 hours after irradiation. Radiograms of 1, 3, 5, and 7 Gy after 24 h of irradiation with 1.5 cGy were 30.7%, 45.1%, 26.4% and 119% respectively. T lymphocytes in children with appropriate low doses of radiation can significantly increase the DNA synthesis, 14h after irradiation 15g 3Gy 3Gy radiation dose-response most fully expressed