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为葡萄霜霉病(Plasmopara viticola)的生物防治提供更多的菌种资源,本研究以新鲜的无病害葡萄叶片为试验材料,通过稀释平板分离法分离得到87株真菌。采用平板对峙法进行初筛得到4株对辣椒疫病菌有拮抗作用的菌株,经过叶盘法复筛得到一株防治效果比较好的Y29菌株,其防治效果达到了72.9%,且防效稳定。通过观察Y29菌株的菌落形态、菌丝形态和产孢结构,分析其26S rDNA D1/D2区序列和系统发育树,鉴定其为小光壳属中的Leptosphaerulina australis。
Which provided more germ resources for the biological control of Plasmopara viticola. In this study, 87 fresh fungal grape leaves were used as experimental materials, and 87 fungi were isolated by dilution plate separation. Four strains of antagonistic fungi against Pepper Phytophthora were screened by the plate confrontation method. One strain Y29 with good control effect was obtained by re-screening by leaf disc method. The control effect was 72.9%, and the control effect was stable. By observing the colony morphology, mycelial morphology and sporulation structure of Y29 strain, the 26S rDNA D1 / D2 region sequence and phylogenetic tree were analyzed and identified as Leptosphaerulina australis.