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我们于1990年,在培养溶组织内阿米巴的过程中,为了延长保种,避免经常传代转种的麻烦,试用液氮冷冻保存溶组织内阿米巴滋养体,并对冻后虫体的生物学特性进行了初步观察。材料与方法一、虫株系上海医科大学寄生虫学教研室提供溶组织内阿来巴B株(北京友谊医院馈赠),以及B株经长爪沙鼠盲肠内接种启传代培养的B_1株。二、冷冻虫体在琼脂-蛋白胨双相培养基中培养2d,取出培养管冰浴5min,收集虫体于无菌离心管,自然沉淀2min,弃去底部粗米粉,再加培养液洗涤悬
In 1990, in the process of culturing Entamoeba histolytica, in order to prolong preservation and avoid the trouble of frequent passages, we try to use cryopreserved solution of Entamoeba histolytica trophozoites in liquid nitrogen, The biological characteristics of a preliminary observation. Materials and methods First, the worm strains Department of Parasitology Shanghai Medical University to provide tissue lysate Nebra B (Beijing Friendship Hospital gift), and B strains of Meriones venitus seeding inoculated Kai passage B 1 strains. Second, the frozen worms cultured in agar - peptone biphasic medium for 2 days, remove the culture tube ice bath 5min, collect the worms in sterile centrifuge tube, natural precipitation 2min, discard the bottom of coarse rice noodles, plus medium washing suspension