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目的研究铅对神经信号传导的关键部位──细胞膜上Ｇ蛋白（Ｇ－ｐｒｏｔｅｉｎｓ）的ｍＲＮＡ表达水平的影响。方法采用胚胎大鼠海马神经元无血清培养方法，加入s不同浓度的氯化铅溶液，设立无铅暴露的空白对照组，采用逆转录多聚酶链反应（ＲＴ－ＰＣＲ）技术半定量测定Ｇ蛋白三种不同类型α亚基，包括Ｇｓ、Ｇｉ２和Ｇｏｌα亚基ｍＲＮＡ表达水平，然后对结果进行相关矩阵分析。结果随着铅暴露水平的提高，Ｇｉ２α、Ｇｏ１αｍＲＮＡ表达水平显著增加，而ＧｓαｍＲＮＡ表达水平却无明显改变。结论铅暴露可干扰海马神经元Ｇ蛋白ｍＲＮＡ的表达，使Ｇ蛋白不同α亚基的ｍＲＮＡ表达水平失去平衡，从而影响神经信号的正常传递。
Objective To study the effect of lead on the expression of G-protein mRNA in the key part of neural signal transduction. Methods Serum-free culture of hippocampal neurons in embryonic rats was used. Different concentrations of lead chloride solution were added to establish a blank control group exposed to lead. Semiquantitative determination of G protein-trisaccharide by three-dimensional reverse transcription polymerase chain reaction (RT-PCR) Different types of α subunits, including Gs, Gi2 and Golα subunit mRNA expression levels, and then the results of correlation matrix analysis. Results With the increase of lead exposure, the expression of Gi2α and Go1α mRNA increased significantly, but the expression of Gsα mRNA did not change significantly. Conclusion Lead exposure may interfere with the expression of G protein mRNA in hippocampal neurons and unbalance the mRNA expression of different α subunits of G protein, thus affecting the normal transmission of neural signals.