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利用定点突变技术获得的ｓＩＬ－６Ｒ突变体Ｈ２８０Ⅰ基因与天然ｓＩＬ－６Ｒ（ｗｔｓＩＬ－６Ｒ）基因分别转染Ｃｏｓ７细胞，转染细胞上清在７ＴＤ１、Ｒ２细胞系上测定生物学活性，并同时进行ＩＬ－６结合能力分析。结果显示，ｓＩＬ－６ＲＨ２８０Ⅰ可以保持同ＩＬ－６的结合能力，同时对ＩＬ－６的信号传递过程起拮抗作用，提示Ｈ２８０的改变可能影响ＩＬ－６Ｒ胞外区与ｇｐ１３０的结合。
The sIL-6R mutant H280Ⅰ gene and the natural sIL-6R (wtsIL-6R) gene obtained by site-directed mutagenesis were transfected into Cos7 cells, and the supernatant of transfected cells were assayed for biological activity on 7TD1 and R2 cell lines simultaneously IL-6 binding ability analysis. The results showed that sIL-6RH280Ⅰ could maintain the binding ability with IL-6 and antagonize the signal transduction of IL-6, suggesting that the change of H280 may affect the binding of IL-6R extracellular domain to gp130.