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本文用ＥＬＩＳＡ间接法检测了一种恶性疟原虫保护性抗原重组复合基因表达产物诱导小鼠体液免疫的反应。该复合基因包含了编码ＭＳＡ１、ＭＳＡ２和ＲＥＳＡ上的Ｔ和／或Ｂ细胞刺激位点的基因片段，同时加有ＩＬ－１和ＴＴ上的广谱Ｔ细胞刺激位点的基因片段。该复合基因在Ｅ．ｃｏｌｉ中的表达产物（简称复合抗原）在有和无佐剂的条件下免疫ＢＡＬＢ／ｃ小鼠后，均产生了明显的抗复合抗原的抗体，同时也产生了明显的抗可溶性裂殖子抗原的抗体。相反，用可溶性裂殖子抗原免疫的ＢＡＬＢ／ｃ小鼠则不能产生明显的抗复合抗原的抗体。另外，经复合抗原免疫的Ｃ５７ＢＬ／６小鼠也能产生明显的抗复合抗原的抗体
In this paper, ELISA indirect method was used to detect a recombinant plasmodium falciparum protective antigen recombinant expression product induced humoral immune response in mice. This complex contains gene fragments encoding T and / or B cell stimulating sites on MSA1, MSA2 and RESA, plus gene fragments of broad-based T-cell stimulating sites on IL-1 and TT. The complex gene in E. The expressed products of E. coli (referred to as the composite antigen) in BALB / c mice immunized with and without adjuvant produced significant anti-composite antigens and also produced significant anti-soluble merozoite antigen Antibody. In contrast, BALB / c mice immunized with soluble merozoite antigens did not produce significant anti-complex antigens. In addition, C57BL / 6 mice immunized with the composite antigen also produced significant anti-complex antigens