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目的利用18氟-氟脱氧葡萄糖(18F-FDG)小动物正电子发射计算机断层显像(micro PET)观察糖尿病小鼠心肌葡萄糖代谢的改变。方法取正常野生型BKS小鼠5只、db/db糖尿病小鼠5只,正常进食,控制氧流量400ml/min,分别在1.5%~1.8%异氟烷麻醉下尾静脉弹丸式注射50~75μl18F-FDG 200~300uci(7.4~11.1MBq)进行micro PET动态显像60min,图像重建后勾画心肌感兴趣区(ROI)获取心肌时间-放射性曲线(TAC),计算心肌葡萄糖标准摄取值(SUV),同时经小鼠尾静脉多点采血获取尾静脉血TAC,利用输入函数数学模型显像分析系统(KIS),计算两组心肌的葡萄糖代谢率(MRGlu),并行t检验。结果与BKS小鼠比较,db/db小鼠显像45~55min SUV、Ki值[(12.92±1.68)vs(5.70±1.19)g/ml,P<0.01],[(0.20±0.05)vs(0.09±0.02)ml/(min·g),P<0.01]降低;平均血糖、葡萄糖代谢率[(6.10±1.31)vs(24.39±3.36)mmol/L,(21.01±2.98)vs(40.14±4.32)mg/(kg·min),P<0.01]升高。结论 18F-FDG micro PET显像可提示db/db糖尿病小鼠心肌葡萄糖摄取及代谢率的改变。
OBJECTIVE: To evaluate the changes of myocardial glucose metabolism in diabetic mice using positron emission tomography (18-F-FDG) micro-positron emission tomography (micro PET). Methods Five normal wild-type BKS mice and five db / db diabetic mice were used. Normal feeding and oxygen flow were controlled at 400ml / min, and caudal vein bolus injection of 50 ~ 75μl18F at 1.5% ~ 1.8% isoflurane anesthesia respectively (FDG) 200 ~ 300uci (7.4 ~ 11.1MBq) for 60min, and then reconstruct the ROI of myocardium to obtain the myocardial time-radiation curve (TAC) after imaging reconstruction. Calculate the myocardial glucose uptake standard (SUV) At the same time, tail vein blood TAC was obtained from the tail vein of mice, and the myocardial metabolic rate (MRGlu) was calculated using the input function mathematical model KIS. The t-test was performed. Results Compared with BKS mice, db / db mice developed SUV at 45-55 min, Ki value [(12.92 ± 1.68) vs (5.70 ± 1.19) g / ml, P <0.01] 0.09 ± 0.02) ml / (min · g), P <0.01]. The average blood glucose and glucose metabolism rate [(6.10 ± 1.31) vs (24.39 ± 3.36) mmol / L and (21.01 ± 2.98 vs 40.14 ± 4.32 ) mg / (kg · min), P <0.01]. Conclusion 18F-FDG micro PET imaging can prompt myocardial glucose uptake and metabolic rate in db / db diabetic mice.