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目的 探讨非淋菌性尿道炎 (NGU)病原体的常规检测方法。方法 报道 46 1份NGU标本用全自动免疫诊断系统 (VIDAS)检测沙眼衣原体 (Chlamydiatrachomatic,CT)。用支原体检测试剂盒 (MycoplasmaIST)检测 10 3份标本中支原体 (UreaplasmaurealytiumUU和MycoplasmahominisMH)的结果。 结果 沙眼衣原体和支原体的检测阳性率分别为 2 0 4%和 34 0 %。支原体阳性检测率男女间有显著差异P <0 0 5。结果还表明CT和支原体感染患者集中于 35岁以下年龄段。支原体药敏试验结果表明 ,对其敏感率最高的药物依次是原始霉素、交沙霉素、强力霉素和四环素。 14份正常人群标本用上述方法均未查出阳性结果。结论 VIDAS和IST具有特异性好、阳性率高、操作方便的优点 ,且非批次性操作可满足门诊、急诊病人立等可取的要求 ,有利于性传播疾病的及时控制。
Objective To investigate the routine detection of non-gonococcal urethritis (NGU) pathogens. Methods 46 cases of NGU specimens were detected by automated immunodiagnostic system (VIDAS) detection of Chlamydia trachomatis (Chlamydiatrachomatic, CT). The results of mycoplasma (Ureaplasma rugalytiumUU and MycoplasmahominisMH) in 10 3 specimens were tested using the Mycoplasma test kit. Results The positive rates of Chlamydia trachomatis and Mycoplasma were 20% and 34% respectively. Mycoplasma positive rate was significantly different between men and women P <0 0 5. The results also indicate that CT and mycoplasma infections are concentrated in people under 35 years of age. Mycoplasma sensitivity test results show that the highest sensitivity of the drug followed by pristinamycin, jasamycin, doxycycline and tetracycline. Fourteen samples of normal population did not find positive results by the above methods. Conclusions VIDAS and IST have the advantages of good specificity, high positive rate and convenient operation, and non-batch operation can meet the requirements of outpatient and emergency patients, which is in favor of the timely control of sexually transmitted diseases.