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目的研究复尔康注射液对肝癌HepG2细胞凋亡和VEGF表达的影响。方法复尔康注射液干预HepG2细胞48 h后,CCK8试剂盒检测细胞的增殖活性,Hoechst 33258荧光染色观察细胞凋亡的形态学改变,流式细胞仪Annexin V/PI双染法检测细胞的凋亡率,免疫组化SABC法检测细胞VEGF的表达。结果复尔康注射液可抑制HepG2细胞的增殖,IC50值为(29.63±1.22)mg/L。复尔康注射液51、0 mg/L干预HepG2细胞48h后,Hoechst 33258荧光染色可见核浓缩、核碎裂等凋亡形态学改变,Annexin V/PI双染法检测显示细胞凋亡率明显升高,免疫组化SABC法检测显示VEGF的表达明显减弱,且呈现出一定的量效关系。结论复尔康注射液可诱导HepG2细胞凋亡,减弱VEGF的表达。
Objective To study the effect of fuerkang injection on the apoptosis of HepG2 cells and the expression of VEGF. Methods Liver cancer cells were exposed to fucomconazole for 48 hours. The proliferation of HepG2 cells was detected by CCK8 kit. The morphological changes of the cells were observed by Hoechst 33258 staining. The apoptosis of HepG2 cells was detected by flow cytometry Annexin V / PI double staining. The rate of apoptosis was detected by immunohistochemical SABC method. Results Fu Er Kang injection can inhibit HepG2 cell proliferation, IC50 value (29.63 ± 1.22) mg / L. The apoptotic rate of HepG2 cells treated with Fuerkang injection 51,0 mg / L for 48h was higher than that of Hoechst 33258 cells, nuclear morphosis and nuclear fragmentation were observed. Annexin V / PI staining showed that the apoptosis rate of HepG2 cells was significantly increased High and immunohistochemical SABC assay showed that the expression of VEGF was significantly weakened, and showed a certain dose-effect relationship. Conclusion Fluconazole injection can induce HepG2 cell apoptosis and decrease the expression of VEGF.