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[目的]探讨2,4-滴丁酯90 d染毒对雄性大鼠学习记忆行为及大脑皮质、海马组织中氨基酸类神经递质含量的影响。[方法]将40只健康30 d龄SPF级雄性Wistar大鼠随机分为4组,分别为对照组(玉米油∶蒸馏水体积比为1∶1)和2,4-滴丁酯低(1/100 LD50,6 mg/kg)、2,4-滴丁酯中(3/100 LD50,18 mg/kg)、2,4-滴丁酯高(6/100 LD50,36 mg/kg)剂量组,每组10只。经口灌胃染毒,每周连续染毒5 d,实验周期为90 d。染毒第4、8、13周进行Morris水迷宫测试大鼠的学习记忆能力。染毒结束后,高效液相色谱法检测大鼠大脑皮质、海马中谷氨酸、天门冬氨酸、γ-氨基丁酸、甘氨酸的含量。[结果]Morris水迷宫测试第13周时,随染毒剂量升高,大鼠平均逃避潜伏期逐渐延长(P<0.05),呈剂量-效应关系(r=0.961 1,P<0.01)。随着2,4-滴丁酯染毒剂量的升高,大鼠大脑皮质中谷氨酸水平呈升高趋势(P<0.05)、γ-氨基丁酸水平呈下降趋势(P<0.05),均呈剂量-效应关系(r=0.942 5,r=-0.887 2,均P<0.01);天门冬氨酸含量在高剂量组升高显著(P<0.05)。海马中谷氨酸含量、天门冬氨酸含量均呈升高趋势(P<0.05)、γ-氨基丁酸含量呈下降趋势(P<0.05),均呈剂量-效应关系(r=0.970 1,r=0.886 6,r=-0.916 2,均P<0.01)。[结论]2,4-滴丁酯亚慢性染毒影响大鼠学习记忆能力及大鼠脑组织氨基酸类神经递质水平。
[Objective] To investigate the effects of 2,4-D butylate 90-day exposure on learning and memory behaviors and amino acid neurotransmitters in cerebral cortex and hippocampus in male rats. [Methods] Forty healthy male Sprague-Dawley (Wistar) rats, 30 days old, were randomly divided into 4 groups: control group (corn oil: distilled water volume ratio of 1: 100 LD50, 6 mg / kg), 2,4-D butyl ester (3/100 LD50, 18 mg / kg) and 2,4-Db (6/100 LD50, 36 mg / , Each group of 10. Oral administration of oral administration, weekly continuous exposure to 5 d, the experimental period of 90 d. At the 4th, 8th and 13th weeks of exposure, the learning and memory abilities of Morris water maze test rats were evaluated. After the exposure, the contents of glutamic acid, aspartic acid, γ-aminobutyric acid and glycine in rat cerebral cortex and hippocampus were detected by HPLC. [Results] At the 13th week of Morris water maze test, the average escape latency of rats increased gradually (P <0.05) with dose-effect relationship (r = 0.961 1, P <0.01). With the increase of 2,4-D butylate exposure, glutamate levels in rat cerebral cortex increased (P <0.05) and γ-aminobutyric acid levels decreased (P <0.05) (R = 0.942 5, r = -0.887 2, both P <0.01). Aspartate content increased significantly in high dose group (P <0.05). The content of glutamic acid and aspartic acid in hippocampus increased (P <0.05) and the content of γ-aminobutyric acid decreased (P <0.05), both showed a dose-effect relationship (r = 0.970 1, r = 0.886 6, r = -0.916 2, all P <0.01). [Conclusion] Subchronic exposure to 2,4-D butyl ester affects learning and memory abilities and levels of amino acid neurotransmitters in rat brain tissue.