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目的 :建立一种囊袋模型 ,研究兔晶体囊外摘除术 (ECCE)后残余的晶体上皮细胞 (LECs)在体外增殖 ,移行 ,化生的情况。方法 :2 0只兔眼 ,体外模拟白内障手术 ,并游离晶体囊袋 ,固定于硅胶环上 ,置含 10 %胎牛血清的DMEM营养液中培养 3周。分别用相差显微镜和光镜观察不同培养时期后囊上LECs的生长情况。结果 :经过 2～ 3天的潜伏期 ,后囊周边部开始出现LECs ,细胞增殖速度较快 ,约 6～ 8天可完全覆盖后囊。组织学可见均质红染的后囊膜上被覆一层或多层排列紧密 核深染胞浆丰富的LECs。结论 :应用这种囊袋模型 ,进行LECs体外培养 ,较为真实的表现了体内ECCE术后的多种变化 ,有助于更深一步探讨后囊膜混浊的发生机制 ,并为筛选防治后发性白内障的有效药物 ,提供了有价值的实验手段
OBJECTIVE: To establish a sachet model to study the proliferation, migration and metaplasia of residual crystal epithelial cells (LECs) after extracapsular cataract extraction (ECCE). Methods: Twenty rabbits were randomly divided into three groups. The cataract surgery was performed in vitro and the capsular bag was free. The cells were fixed on silica gel and cultured in DMEM containing 10% fetal bovine serum for 3 weeks. The growth of LECs in the posterior capsule was observed by phase contrast microscopy and light microscopy. Results: After 2 to 3 days of incubation, LECs began to appear in the periphery of the posterior capsule. The proliferation rate of the LECs was very fast. The posterior capsule could be completely covered in about 6 to 8 days. Histology can be seen homogeneous red stained posterior capsule coated with one or more layers arranged closely nuclear deep-dyeing cytoplasm-rich LECs. CONCLUSIONS: Using this sachet model to culture LECs in vitro, it is a real demonstration of many changes after ECCE in vivo and is helpful to further explore the mechanism of posterior capsule opacification. In order to screen for the prevention and treatment of cataract The effective drug, provides a valuable experimental means