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目的研究P53、Ki-67、PCNA在宫颈鳞状上皮不典型增生、鳞状上皮原位癌及浸润性鳞状上皮癌中的表达及意义。方法用免疫组化方法(S-P法)检测9例慢性宫颈炎、6例尖锐湿疣、24例宫颈鳞状上皮不典型增生、13例原位癌、5例早期浸润癌和33例浸润性宫颈鳞状上皮癌P53、Ki-67、PCNA的表达情况。结果P53在慢性宫颈炎,宫颈尖锐湿疣,不典型增生,原位癌及浸润性鳞状上皮癌之间无明显差异性,但与癌组织分化程度有关(P<0·05)且早期浸润癌与浸润性鳞癌之间差异非常显著(P<0·01),Ki-67的表达与组织分化程度有关(P<0·05),PCNA在慢性宫颈炎中无明显表达,在尖锐湿疣、不典型增生及癌中均有93·3%~100%的表达,且与组织分化程度无关。结论P53异常表达是宫颈癌的早期事件,与宫颈癌发生有关,PCNA和Ki-67在宫颈鳞状上皮不典型增生及癌中均有高表达,表明它们在宫颈鳞状上皮癌的发生及发展中起作用。
Objective To study the expression and significance of P53, Ki-67 and PCNA in cervical squamous cell atypical hyperplasia, squamous cell carcinoma in situ and invasive squamous cell carcinoma. Methods Nine cases of chronic cervicitis, 6 condyloma acuminatum, 24 cases of atypical hyperplasia of cervical squamous epithelium, 13 cases of carcinoma in situ, 5 cases of early invasive carcinoma and 33 cases of invasive cervical squamous cell carcinoma were detected by immunohistochemistry (SP method) Like epithelial carcinoma P53, Ki-67, PCNA expression. Results There was no significant difference in P53 between chronic cervicitis, cervical condyloma acuminata, atypical hyperplasia, carcinoma in situ and invasive squamous cell carcinoma, but it was related to the degree of differentiation (P <0.05) and early invasive carcinoma (P <0.01). The expression of Ki-67 was correlated with the degree of tissue differentiation (P <0.05). There was no significant difference in the expression of PCNA between chronic cervicitis and condyloma acuminatum, Atypical hyperplasia and cancer were 93 · 3% to 100% of the expression, and has nothing to do with the degree of tissue differentiation. Conclusions The abnormal expression of P53 is an early event of cervical cancer and is related to the occurrence of cervical cancer. The expressions of PCNA and Ki-67 are both highly expressed in cervical squamous dysplasia and carcinoma, indicating their occurrence and development in cervical squamous cell carcinoma In work.