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为了提高家蚕抗病力的功效,作者于1962年进行了紫外线照射家蚕的试验。初步肯定,照射能提高家蚕幼虫期对核型多角体病毒感染的抵抗力。今将试验结果整理于后,供作参考。 方法及材料 用家蚕华九×瀛汉一代杂种作为材料。自丙_2胚子期催青开始,在紫外线灭菌灯下照射直到点青期止。紫外线灭菌灯波长3537埃,照射距离45厘米,照射时间第一次在春季,分为每日0、15、30、60和90分钟五个等级。第二次在秋季分为0、30、60、90、120和150分钟六个等级。第三次重复试验照射90分钟与未照射二个区。各级试验区的蚕卵孵化后饲养到三龄超蚕时,用核型多角体病毒LD(50)的剂量(每头30万粒)饲喂幼虫,测定幼虫对核型多角体病毒抵抗力提高的程度。
In order to improve the effectiveness of silkworm disease resistance, the author carried out in 1962 experiments UV irradiation silkworm. It is initially affirmed that irradiation can improve the resistance to nuclear polyhedrosis virus infection in the larval stage of silkworm. This test results will be sorted out for reference. Methods and materials Silkworm Hua IX × Ying Han generation hybrids as a material. From C 2 embryonic stage of greening began, under UV light irradiation until the point of youth only. UV sterilizing light wavelength 3537 angstroms, the irradiation distance of 45 cm, the first time in the spring, divided into five levels daily 0,15,30,60 and 90 minutes. The second time in autumn is divided into six levels of 0, 30, 60, 90, 120 and 150 minutes. The third repeated test irradiation for 90 minutes with no irradiation of two districts. When silkworm eggs of all levels were hatched and fed to the third instar larva, larvae were fed with a dose of 300000 nucleotides of polyhedrosis virus (LD (50)), and the larvae resistance to nuclear polyhedrosis virus The degree of improvement.