论文部分内容阅读
鲤科是世界鱼类中最大的科,有210余属2010种.为研究其系统发育关系需要筛选合适的DNA标记.将S7核糖体蛋白基因用作遗传标记进行鲤科鱼类的系统发育分析.通过PCR方法,扩增了长度为602bp的胭脂鱼以及长度为655～859bp的16种鲤科鱼类的S7基因内含子1序列.序列排列得到925个排列位点,其中信息位点499个,占全部位点的54％.结果表明,鲤科鱼类S7基因内含子1序列具有丰富的信息位点,并且在亲缘关系不同的物种间存在显著的序列差异.基于S7基因内含子1序列的NJ（neighbor-joining）和MP（most-parsimony）分支树经1000次重复抽样检验后,节点的自展支持率普遍高于以细胞色素b及线粒体控制区（d-loop）基因为遗传标记时所得到的分支树.因此,S7基因内含子1作为遗传标记在鲤科系统发育研究中的分辨率是比较高的,它是一个适合鲤科内亚科水平系统发育研究的有用的分子遗传标记.但S7基因内含子1是否适用于鲤形目科间或科以上水平的分子系统学分析,有待进一步研究.
Cyprinidae is the world’s largest family of fish, there are more than 210 genera and 2010 species. To study the phylogenetic relationship need to screen for suitable DNA markers. The S7 ribosomal protein gene as a genetic marker phylogenetic analysis of carps The sequence of intron 1 of S7 gene was amplified by PCR from the longissimus dorsi (602 bp) and 16 species of carps (655-859 bp in length). The alignment of the sequences revealed 925 sequences with 499 informative sites , Accounting for 54% of the total loci.The results showed that the sequence of intron 1 of the S7 gene of the carp was rich in informative sites and there were significant sequence differences among species with different genetic relationships.Based on the intron S7 1 neighbor-joining (MP) and MP (most-parsimony) branch trees after 1000 repeated sampling tests, the self-spreading support rate of nodes was generally higher than that of the cytochrome b and d-loop genes Genetic markers of the branch tree.Therefore, S7 gene intron 1 as a genetic marker in Cyprinidae phylogenetic studies of the resolution is relatively high, it is a suitable for Cyprinidomycetes phylogenetic studies of the level of useful Molecular genetic markers. However, whether intron 1 of S7 gene is suitable for molecular phylogenetic analysis among Cypriniformes or above families remains to be further studied.