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应用超离心和柱层析结合聚丙烯酰胺凝胶电泳（ＰＡＧＥ）及电镜技术，研究中国鲎血蓝蛋白的结构特点．结果表明，经ＳｅｐｈａｄｅｘＧ－１００纯化的血蓝蛋白在ＰＡＧＥ电泳中出现４条带．纯化的血蓝蛋白再经ＤＥＡＥ－３２纤维素柱层析得到５个洗脱峰，每个峰在ＰＡＧＥ电泳下可分辨出４条带．在电镜下，血蓝蛋白分子出现环形、五角形、十字形和蝴蝶结形等构型并与其他解离的中间的构型同时存在．
The structural characteristics of Chinese haemocyanin were studied by ultracentrifugation and column chromatography combined with polyacrylamide gel electrophoresis (PAGE) and electron microscopy. The results showed that there were 4 bands in PAGE electrophoresis of hemocyanin purified by SephadexG-100. Purified hemocyanin and then by DEAE-32 cellulose column chromatography to obtain five eluting peaks, each peak can be resolved by PAGE electrophoresis 4 bands. Under electron microscopy, the hemocyanin molecules appear in conformations such as rings, pentagons, crosses, and bowknots, and co-exist with other dissociated intermediate configurations.