论文部分内容阅读
目的 :观察葛根素注射液在小鼠肠缺血再灌注肝损伤中的抗氧化作用。方法 :制作小鼠肠缺血再灌注肝损伤模型 ;小鼠缺血时间 2 0min ,再灌流 1h后制备血清 ,取肝脏组织观察病理学改变 ,余制成肝匀浆 ,测定SOD活性和MDA含量。结果 :不同剂量的葛根素注射液可减轻小鼠肠缺血再灌注肝损伤的病理学损害 ,并使肝脏SOD活性和MDA含量几乎恢复正常。结论 :葛根素注射液对小鼠肠缺血再灌注肝损伤具有保护作用 ,其肝保护作用机理与其抗氧化作用有关。
Objective: To observe the anti-oxidation effect of puerarin injection in hepatic injury induced by intestinal ischemia-reperfusion in mice. Methods: The model of hepatic injury induced by intestinal ischemia-reperfusion in mice was established. The time of ischemia was 20 min. After the rats were perfused for 1 h, the serum was prepared. The liver tissue was taken for observing the pathological changes. The liver homogenate was prepared and the SOD activity and MDA content were measured. . RESULTS: Different doses of puerarin injection could alleviate the pathological damage of hepatic injury induced by intestinal ischemia and reperfusion in mice, and the SOD activity and MDA content in the liver almost returned to normal. Conclusion :Puerarin has a protective effect on liver injury induced by intestinal ischemia and reperfusion in mice. Its protective mechanism of liver protection is related to its antioxidation.