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目的探讨膜型基质金属蛋白酶-1(MT1-MMP)蛋白表达在乳腺癌发生、发展中的临床病理意义。方法收集2011年1月至2014年12月404例女性乳腺组织石蜡标本分为5组,其中浸润性乳腺癌组220例、导管原位癌(DCIS)Ⅲ组39例、DCISⅠ~Ⅱ组33例、普通型导管增生(UDH)组64例、正常乳腺组48例。采用免疫组化En Vision法检测MT1-MMP蛋白的表达。结果 (1)浸润性乳腺癌、DCISⅢ、DCISⅠ~Ⅱ、UDH、正常乳腺组中MT1-MMP蛋白阳性率分别为67.73%(149/220)、25.64%(10/39)、6.06%(2/33)、4.69%(3/64)、18.75%(9/48);浸润性乳腺癌组阳性率显著高于DCISⅢ、DCISⅠ~Ⅱ、UDH和正常乳腺组(均P<0.01);DCISⅢ组阳性率显著高于DCISⅠ~Ⅱ和UDH组(均P<0.05)。(2)MT1-MMP蛋白表达与腋窝淋巴结转移、TNM分期及孕激素受体(PR)相关(均P<0.05)。log is tic回归模型多因素分析显示浸润性乳腺癌患者MT1-MMP蛋白表达的独立相关因素包括腋窝淋巴结转移(OR=0.213,95%CI=0.084~0.544,P<0.01)和PR表达状态(OR=1.927,95%CI=1.049~3.542,P<0.05)。结论 MT1-MMP蛋白表达自UDH和DCISⅠ~Ⅱ、DCISⅢ至浸润性乳腺癌显著升高,且其MT1-MMP蛋白表达与浸润性乳腺癌患者多项预后不良因素相关,提示其在乳腺癌进展的全过程中均发挥了重要作用,有望成为抗乳腺癌治疗的一个潜在生物学标志。
Objective To investigate the clinicopathological significance of the expression of membrane-type matrix metalloproteinase-1 (MT1-MMP) in the development and progression of breast cancer. Methods From January 2011 to December 2014, 404 cases of female breast tissue paraffin specimens were divided into five groups, including 220 cases of invasive breast cancer, DCIS Ⅲ group 39 cases, DCIS Ⅰ ~ Ⅱ group of 33 cases , Common ductal hyperplasia (UDH) group of 64 cases, normal breast group of 48 cases. Immunohistochemical En Vision method was used to detect the expression of MT1-MMP protein. Results The positive rates of MT1-MMP protein in invasive breast cancer, DCISⅢ, DCISⅠ-Ⅱ, UDH and normal breast tissues were 67.73% (149/220), 25.64% (10/39) and 6.06% (2 / The positive rate of invasive breast cancer was significantly higher than that of DCISⅢ, DCISⅠ-Ⅱ, UDH and normal breast (all P <0.01); DCISⅢgroup was positive The rates were significantly higher in DCIS Ⅰ ~ Ⅱ and UDH groups (all P <0.05). (2) MT1-MMP protein expression was correlated with axillary lymph node metastasis, TNM stage and progesterone receptor (PR) (all P <0.05). log is tic regression model multivariate analysis showed that the independent relevant factors of MT1-MMP protein expression in invasive breast cancer include axillary lymph node metastasis (OR = 0.213, 95% CI = 0.084-0.544, P <0.01) = 1.927, 95% CI = 1.049 ~ 3.542, P <0.05). Conclusion MT1-MMP protein expression was significantly increased from UDH, DCISⅠ-Ⅱ and DCISⅢ to invasive breast cancer, and MT1-MMP protein expression was correlated with a number of poor prognostic factors in patients with invasive breast cancer, suggesting that MT1- The whole process has played an important role, is expected to become a potential biological markers of anti-breast cancer treatment.