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将供试新疆棉花枯萎病菌32个代表菌株及3个标准菌株进行RAPD分析,筛选出14个随机引物,共产生252个RAPD分子标记,其中79.4％具有多态性。通过聚类分析将供试菌株划分为5个RAPD组,此结果与常规鉴别寄主反应法划分生理小种的结果基本一致,RAPD分析为传统方法的结果提供了分子水平的证据,从分子水平上证明新疆棉花枯萎菌群体内7号小种占绝对优势。RAPD可作为一种标记手段用于棉花枯萎病菌生理小种及遗传分化的检测。
RAPD analysis of 32 representative strains and 3 standard strains of Fusarium oxysporum f. Sp. In Xinjiang was carried out. Fourteen random primers were screened and a total of 252 RAPD markers were generated, of which 79.4% were polymorphic. The result of cluster analysis was divided into five RAPD groups. The results were basically the same as the results of conventional identification of the host-reaction races. RAPD analysis provided evidence of the molecular level for the results of traditional methods. It was proved that the No. 7 races of cotton fescue in Xinjiang accounted for the absolute predominance. RAPD can be used as a marker for the detection of physiological races and genetic differentiation of Fusarium oxysporum f.