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目的:研究三七总皂甙(PNS)对减轻脑损伤后脑组织继发性损害的机理。方法:参照王荣丽方法检测家兔脑损伤后24h脑组织NO含量,改良八木国夫法检测脑组织丙二醛(MDA)含量,干湿法测定脑组织含水量,观察PNS对上述指标的影响。结果:脑损伤24h脑损伤组NO、MDA及含水量均高于脑损伤+PNS组和对照组,p<0.01。同时,脑组织中NO和MDA含量与含水量均呈显著正相关,p<0.01。脑损伤+PNS组和对照组脑组织中NO、MDA及含水量均无显著性差异。结论:PNS能通过降低脑损伤后NO及MDA的产生,减轻脑水肿的发生,对继发性脑组织损伤具有一定保护作用。
OBJECTIVE: To study the mechanism of total saponins of Panax notoginseng (PNS) on relieving brain damage following brain injury. Methods: The content of NO in brain tissue was determined 24 h after brain injury in rabbits with reference to Wang Rongli method. The malondialdehyde (MDA) content in brain tissue was detected by modified Yagi-Nagao method. The water content of brain tissue was measured by dry-wet method. The effects of PNS on the above indexes were observed. RESULTS: NO, MDA, and water content in brain injury group were higher than those in brain injury + PNS group and control group after 24h brain injury, p<0.01. At the same time, there was a significant positive correlation between NO and MDA content and water content in brain tissue, p<0.01. There was no significant difference in NO, MDA and water content in brain tissue between brain injury + PNS group and control group. Conclusion: PNS can reduce the occurrence of brain edema by reducing the production of NO and MDA after brain injury, and has a protective effect on secondary brain tissue damage.