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目的研究脐血血管紧张素转换酶(ACE)基因多态性与胎膜早破的相关性。方法收集2008年4月至2008年11月份在内蒙古妇幼保健院产科分娩的产妇脐带血,设胎膜早破组为实验组,正常足月分娩组为对照组,实验组94例,对照组118例,取脐静脉血3ml,经EDTA抗凝,分别提取基因组DNA,然后用ACE引物进行聚合酶链反应(PCR),对扩增产物进行电泳,测定ACE基因型,分别为DD、ID和II 3种基因型。数据统计分析应用SPSS13.0版统计软件进行统计学分析。结果实验组中ACE基因DD型和D等位基因分布频率明显高于对照组,与对照组比较,P<0.05差异显著。对照组ACE基因型以II型为主,与实验组比较,P<0.05差异显著。ID型在两组间分布频率比较P>0.05,差异无显著性。结论脐血ACE基因多态性与胎膜早破具有密切相关性。
Objective To investigate the relationship between cord blood angiotensin converting enzyme (ACE) gene polymorphism and premature rupture of membranes. Methods Maternal umbilical cord blood was collected from April 2008 to November 2008 in the obstetric delivery of MCH in Inner Mongolia. The premature rupture of membranes group was taken as the experimental group, and the normal term delivery group as the control group. The experimental group was 94 cases and the control group 118 For example, taking 3 ml of umbilical blood and EDTA anticoagulant to extract genomic DNA respectively, and then carrying out polymerase chain reaction (PCR) with ACE primers, amplifying the products for electrophoresis and determining ACE genotypes, namely DD, ID and II Three genotypes. Statistical analysis of data SPSS13.0 statistical software for statistical analysis. Results The frequency of DD genotypes and D allele of ACE gene in the experimental group was significantly higher than that in the control group, P <0.05 significant difference compared with the control group. The ACE genotype of the control group was mainly type II, P <0.05 significant difference compared with the experimental group. ID type distribution frequency between the two groups P> 0.05, the difference was not significant. Conclusion The cord blood ACE gene polymorphism is closely related with premature rupture of membranes.