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目的测定转化生长因子β1(transfer growth factor betal,TGF-β1)在接尘工人血清中的表达水平,探讨其作为反映接尘暴露及尘肺早期诊断指标的可能性。方法采用双抗体夹心法,测定72例煤矿接尘工人(分为无尘肺0+期组32人与接尘组40人)和45例健康对照者血清TGF-β1的表达水平。结果无尘肺0+者血清TGF-β1含量为(59.71±7.74)ng/ml、接尘组为(48.45±5.45)ng/ml,对照组为(13.89±2.40)ng/ml。0+期组、接尘组血清TGF-β1含量均高于对照组,差异有统计学意义(P<0.05),0+期组与接尘组差异亦有统计学意义,0+期组与接尘组按工龄分层,各组内层之间差异无统计学意义。结论本次研究提示,TGF-β1可以作为监测粉尘暴露的指标,亦可做为早期筛查高危人群的指标。
Objective To determine the expression level of transforming growth factor β1 (TGF-β1) in sera from workers exposed to dust, and to explore the possibility of TGF-β1 as an early indicator of dust exposure and pneumoconiosis. Methods Double antibody sandwich method was used to detect the expression of TGF-β1 in sera of 72 coal mine workers (divided into 32 in dust-free group 0 and 40 in dust-receiving group) and 45 healthy controls. Results The levels of TGF-β1 in serum-free lungs were (59.71 ± 7.74) ng / ml, (48.45 ± 5.45) ng / ml in the dust-free group and (13.89 ± 2.40) ng / ml in the control group) Serum levels of TGF-β1 in 0 + group and control group were significantly higher than those in control group (P <0.05), but there was also a significant difference between 0 + group and Dian- According to the length of service stratified by age group, there was no significant difference between the inner layers of each group. Conclusion This study suggests that TGF-β1 can be used as an indicator of dust exposure and as an indicator of early screening of high-risk populations.