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目的探讨CUB结构域蛋白1(CUB domain-containing protein 1,CDCP1)在肺癌中的表达及临床意义。方法肺癌患者78例,其中40例采用Western blot方法检测肺癌及手术切缘癌旁组织中CDCP1蛋白表达;应用免疫组织化学法检测78例肺癌及切缘组织病理切片中CDCP1的表达,并分析其与肺癌生物学行为的关系。结果肺癌组织中CDCP1高表达率(67.5%)高于癌旁组织(32.5%),随TNM分期增加,CDCP1在肺癌组织中表达量逐渐增加(P<0.05);免疫组织化学结果显示,CDCP1在肺癌组织中高表达率(57.7%)高于癌旁组织(14.1%)(P<0.05);有淋巴结转移者CDCP1表达率(67.3%)高于无淋巴结转移者(38.5%)(P<0.05);CDCP1表达与肺癌患者生存期呈负相关(r=0.804,P=0.031);Cox分析显示TNM分期(OR=1.216,95%CI:0.649~5.525,P=0.012)和CDCP1(OR=0.526,95%CI:0.153~0.426,P=0.009)是影响肺癌患者预后的独立因素。结论 CDCP1可能是肺癌多阶段演变过程中的重要变化分子之一,可能成为肺癌分子诊断的生物标志物。
Objective To investigate the expression of CUB domain-containing protein 1 (CDCP1) in lung cancer and its clinical significance. Methods 78 cases of lung cancer patients, of which 40 cases of Western blot was used to detect the expression of CDCP1 protein in lung cancer and adjacent tissues adjacent to the surgical margin. Immunohistochemistry was used to detect the expression of CDCP1 in 78 cases of lung cancer and its margins. And biological behavior of lung cancer. Results The high expression rate of CDCP1 in lung cancer tissues was higher than that in adjacent tissues (67.5% vs. 32.5%, P <0.05). The expression of CDCP1 in lung cancer tissues was increased with the increase of TNM stage (P <0.05). The results of immunohistochemistry The positive rate of CDCP1 in lung cancer was higher than that in adjacent non-cancerous tissues (57.7% vs 14.1%, P <0.05) ; The expression of CDCP1 was negatively correlated with the survival of patients with lung cancer (r = 0.804, P = 0.031). The Cox analysis showed that the expression of CDCP1 was positively correlated with the survival of patients with lung cancer (OR = 1.216,95% CI: 0.649-5.525, P = 0.012) 95% CI: 0.153-0.426, P = 0.009) were independent prognostic factors in patients with lung cancer. Conclusion CDCP1 may be one of the most important molecules in the multi-stage evolution of lung cancer and may be a biomarker for the molecular diagnosis of lung cancer.