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利用本室建立的大鼠气管上皮（ＲＴＥ）细胞转化体系，研究了吸烟和肺癌发生的关系以及异硫氰酸衍生物ＰＨＩＴＣ对吸烟诱发肺癌的防护作用。结果表明，香烟烟雾凝集物及其中的亚硝胺ＮＮＫ可以引起ＲＴＥ细胞的转化。采用改进的气管滴注致癌物的方式，较以往降低了ＮＮＫ引起转化的最低济量，有利于正确评估香烟诱发肺癌的危险性。异硫氰醉衍生物ＰＨＩＴＣ可以抑制ＮＮＫ诱导的ＤＮＡ加成物的形成及ＮＮＫ对ＲＴＥ细胞的转化作用。
Using the rat tracheal epithelium (RTE) cell transformation system established in this laboratory, the relationship between smoking and lung cancer development and the protective effect of isothiocyanate derivative PHITC on smoking-induced lung cancer were studied. The results showed that cigarette smoke agglutinates and their nitrosamines NNK can cause RTE cell transformation. The use of improved tracheal instillation of carcinogens reduces the amount of NNK-induced transformation to a lower level than in the past, which is conducive to a correct assessment of the risk of cigarette-induced lung cancer. The isothiocyanate derivative PHITC inhibits the formation of NNK-induced DNA adducts and the effect of NNK on RTE cell transformation.