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以肿瘤为终点的体内长期诱癌实验,所需时间长,人力、物力花费大。近来,国外许多研究者根据癌症形成两阶段理论,探索和建立多种致肝癌作用体内短期实验模型,这类实验模型,以肝细胞增生性病变和r-谷氨酰转肽酶染色阳性肝细胞灶(r-GT灶)为观察指标,仅需数周至数月;肝细胞增生性病变和r-GT灶被认为是肝细胞癌的癌前病变,和肝细胞癌有明显的相应关系,短期实验结果和长期实验结果相一致。应用这种实验模型,不仅可以从形态学、生物化学、生物学行为等方面对肝癌形成的发动和促进阶段以及各阶段的不同步骤
The tumor-terminated long-term cancer-inducing experiment in the body requires a long time and requires a lot of manpower and material resources. Recently, many foreign researchers have explored and established various in vivo short-term experimental models of hepatocarcinogenesis based on the two-stage theory of cancer formation. These experimental models used hepatocyte proliferative lesions and r-glutamyl transpeptidase to stain positive hepatocytes. Lesion (r-GT) is an observational indicator, and it only takes weeks to months; hepatic proliferative lesions and r-GT lesions are considered precancerous lesions of hepatocellular carcinoma, and there is a clear corresponding relationship with hepatocellular carcinoma, short-term The experimental results are consistent with the long-term experimental results. Using this experimental model, not only the stages of initiation and promotion of liver cancer formation, but also the different steps of each stage, from morphology, biochemistry, biological behavior, etc.