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从晶体蛋白的修饰入手,研究了Wistar大鼠晶体蛋白的结构与功能的动态变化及其与老年白内障形成的关系,并对复方参果口服液预防老年性白内障的发生机理和药物防治老年性白内障、延缓衰老的整体效应作了深入的研究。结果表明:Wistar大鼠晶体、晶体核的重量、晶体蛋白α、β大分子组分及晶体蛋白糖苷化水平,都随着年龄的增长而增加;而晶体皮层与核的重量比、晶体蛋白γ小分子组分与晶体蛋白SOD活性均随年龄增长而降低。老龄大鼠经复方参果口服液治疗后(从18月龄给药至24月龄),使老龄大鼠晶体蛋白糖苷化水平明显降低,使肝组织SOD活性显著提高,能明显降低给药组老龄大鼠白内障发生率。整体研究结果表明:复方参果口服液能促进老龄大鼠骨髓有核细胞DNA合成代谢,提高DNA核酸含量,明显提高DNA聚合酶α活力,能显著降低老龄大鼠脑组织乙酰胆硷含量,抗中枢神经系统老化,改善脂类代谢,降低胆固醇,减少尾部老年斑块的形成。这些整体效应生化指标的变化与晶体蛋白的修饰及糖苷化作用水平呈规律性的相互印证,即这些变化有利于机体生命活力的提高,延缓衰老,减少老年性白内障的发生。
Starting from the modification of crystal protein, the dynamic changes of the structure and function of the crystal protein of Wistar rat and its relationship with the formation of senile cataract were studied. The mechanism of the prevention of senile cataract and the prevention and treatment of senile cataract were also studied. The overall effect of delaying aging has been studied in depth. The results showed that the weight of crystals, crystal nucleus, crystal protein α, β macromolecule components and crystal protein glycosylation of Wistar rats all increased with age; while the weight ratio of crystal cortex to nucleus, crystal protein γ Both small molecule components and crystal protein SOD activity decreased with age. Aging rats after treatment with Compound Shenshen Oral Liquid (from 18 months of age to 24 months of age) significantly reduced the crystal protein glycosylation in old rats, significantly increased SOD activity in the liver, and significantly reduced the dosage of the administration group. Incidence of cataract in aged rats. The overall results showed that: Compound Shenuo Oral Liquid can promote DNA synthesis and metabolism of bone marrow nucleated cells in aged rats, increase DNA nucleic acid content, significantly increase the DNA polymerase alpha activity, can significantly reduce the brain tissue acetylcholine content in aged rats, resistance The aging of the central nervous system improves lipid metabolism, lowers cholesterol, and reduces the formation of senile plaques in the tail. These changes in the overall effect of biochemical indicators and the level of crystal protein modification and glycosylation are regular evidence that these changes are conducive to the improvement of vitality of the body, delay aging and reduce the incidence of senile cataract.