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一、简史在1901年法国诺卡尔(Nocard)氏从牛乳中分离出一株牛型结核菌,保存在实验室内,后经法国医生卡美德(Calmette)和兽医介兰(Guèrin)二氏用含牛胆汁的马铃薯培养基进行培养。他们在试验过程中,每间隔2周将此菌株传种一代,到第15代时,把达100毫克结核菌注入到静脉内,亦未见豚鼠死亡。至第33代注射大量的活菌到犊牛(小牛)体内,亦不能引起疾病,又经过一段时期,在注射过这种活菌的犊牛再注射毒力
A Brief History In 1901 Nocard of France isolated from cattle milk in a strain of M. tuberculosis, stored in the laboratory, after the French doctors Calmette and veterinary medlar (Guèrin) two Culture with potato bovine-containing medium. During the course of the trial, they transmitted the strain at intervals of two weeks. By the 15th generation, 100 mg of TB were injected into the vein and no guinea pigs died. By the 33rd generation, large quantities of viable cells are injected into calves (calves) and can not cause disease. After a period of time, the calves injected with this viable bacteria are injected with virulence again