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目的：观察海洛因依赖者外周血T淋巴细胞核仁形成区（NORs）酸性非组蛋白（Ag-NORs）表达及与解毒有关的胎盘型谷胱甘肽S-转移酶（CST-π）表达，分析海洛因依赖对T细胞代谢乃至功能的影响。方法：利用北京开隆公司提供的KL型免疫图象分析系统检测T细胞Ag-NORs；通过定量PCR技术分析病人外周血GST-π。表达。结果：72例海洛因依赖者外周血T淋巴细胞Ag-NORs检测平均值6．78％±0.50％（％±S），其中33例进行了GST-π表达分析，与正常人比较海洛因依赖者外周血GST-π表达明显增高，且Ag－NORs与GST-π表达呈明显负相关。结论：海洛因依赖者外周血T淋巴细胞Ag-NORs明显降低，表明其免疫细胞功能受到明显抑制。Ag-NORs降低的幅度明显小于肿瘤患者，说明海洛因依赖对T细胞的抑制可能在途径或机理上有别于肿瘤抑制因子对免疫细胞的抑制。GST-π表达增高，提示机体损伤修复机理加强。CST-π表达与Ag-NORs呈密切负相关，进一步证实海洛因依赖患者免疫功能损伤与修复对立调节的加强。
Objective: To observe the expression of acidic non-histone proteins (Ag-NORs) and the expression of placental glutathione S-transferase (CST-π) related to detoxification in the peripheral blood of heroin dependent patients. Effects of heroin dependence on T cell metabolism and even its function. Methods: Ag-NORs of T cells were detected by KL type immuno-image analysis system provided by Beijing Kailong Company. GST-π of peripheral blood was analyzed by quantitative PCR. expression. Results: The mean value of Ag-NORs in 72 cases of heroin addicts was 6.78% ± 0.50% (% ± S), of which 33 cases were analyzed by GST-π expression. Compared with normal subjects, the peripheral The expression of GST-π in blood was significantly increased, and the expression of Ag-NORs was negatively correlated with the expression of GST-π. Conclusion: Ag-NORs in peripheral blood T lymphocytes of heroin addicts were significantly decreased, indicating that immune cells function was significantly inhibited. Ag-NORs decreased significantly less than cancer patients, indicating that heroin dependence on T-cell inhibition may be different in pathway or mechanism of tumor suppressor on immune cells. GST-π expression increased, suggesting that the body injury repair mechanism strengthened. CST-πexpression was negatively correlated with Ag-NORs, further confirming the strengthening of contralateral immune regulation and repair in patients with heroin dependence.