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目的:比较人恒牙牙髓、牙乳头组织中β连环蛋白(β-catenin)的mRNA和蛋白的表达情况,探讨β-catenin与牙髓牙本质复合体发育之间的关系。方法:收集48颗因正畸而拔除的健康前磨牙,拔除后立即取出牙髓和牙乳头组织。实验分为3组,第1组为年轻恒牙的牙乳头组织,第2组为年轻恒牙的牙髓组织,第3组为成熟恒牙的牙髓组织。用RT-PCR及Western印迹检测β-catenin的表达,图像分析软件(Meta Morph)对阳性条带进行灰度值的测定。采用SPSS11.0软件包对数据进行两样本t检验。结果:β-catenin在年轻恒牙的牙乳头、牙髓及成熟恒牙的牙髓组织中均有mRNA和蛋白表达,在年轻恒牙牙髓组织中的表达强于在牙乳头组织以及成熟恒牙牙髓组织中的表达(P<0.05)。结论:β-catenin在年轻恒牙和成熟恒牙的牙乳头、牙髓组织中均有不同程度的表达,提示β-catenin在牙髓牙本质复合体发育及牙髓损伤后的修复过程中可能发挥重要作用。
OBJECTIVE: To compare the mRNA and protein expression of β-catenin in dental pulp and dental papilla, and to explore the relationship between β-catenin and the development of dental pulp dentin complex. Methods: Forty-eight healthy premolar teeth were collected from orthodontics, and the pulp and dental papilla tissues were removed immediately after removal. The experiment was divided into three groups, the first group was the dental papilla tissue of young permanent teeth, the second group was the pulp tissue of young permanent teeth, and the third group was the pulp tissue of mature permanent teeth. The expression of β-catenin was detected by RT-PCR and Western blot, and the gray value of the positive band was determined by image analysis software (Meta Morph). SPSS11.0 software package for the two samples t test. Results: The mRNA and protein expression of β-catenin in the dental pulp of young permanent teeth, dental pulp and mature permanent teeth were stronger than those in dental pulp tissue and mature permanent teeth Expression in myeloid tissue (P <0.05). CONCLUSION: The expression of β-catenin in the dental papilla and pulp tissue of young permanent and mature permanent teeth are different, suggesting that β-catenin may play an important role in the development of pulp dentin complex and the repair of dental pulp injury Play an important role.