作者分别用埃及和曼氏血吸虫的尾蚴接种金色仓鼠,感染50天后解剖,收集肝与肠系膜静脉内的成虫,洗净,制成匀浆,冰冻、离心、将上清液透析、抽干,分别制成成虫抗原。与埃及血吸虫病患者、曼氏血吸虫病患者和混合感染患者血清进行酶标记试验。试验方法参照Engvall(1972)塑料管包埋抗原法进行。 The authors inoculated golden hamsters with cercariae of Schistosoma mansoni, respectively, and dissected 50 days after infection. The adults in the liver and mesenteric vein were collected, washed, homogenized, frozen and centrifuged. The supernatant was dialyzed and drained, Made of adult antigens. Enzyme-labeled assays were performed with serums from patients with schistosomiasis japonica, patients with Schistosoma mansoni, and patients with mixed infections. The test method is based on Engvall (1972) plastic tube embedding antigen method.