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为探讨冬季叶色变化原因及其与抗氧化的关系,本文以黄绿色和灰绿色变异类型的沙地柏为研究对象,观测叶色及色素含量、受胁迫程度和抗氧化系统等生理指标的变化。结果表明,在自然越冬过程中,黄绿色的叶色由黄绿色转为橙红色,灰绿色的由绿色变为灰绿或灰色;与此同时,黄绿色叶片的花色素苷(Acy)显著升高,叶绿素(Chl)及类胡萝卜素(Car)含量变化不明显,灰绿色叶片的Chl含量显著降低,Car及Acy含量显著升高。在反应植物受胁迫程度的指标中,过氧化氢(H2O2)、相对电导率(EL)及丙二醛(MDA)的含量在入冬后逐渐升高,次年1或2月份达到最高,之后下降;最大光化学效率(Fv/Fm)则表现为相反的变化趋势,次年1月份最低。在抗氧化系统中,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸(As A)及黄绿色变异类型中的还原型谷胱甘肽(GSH)呈现与H2O2相似的变化趋势,而灰绿色中的GSH含量则呈现高-低-高-低的变化趋势。相关性分析结果表明,两种变异类型的沙地柏色素和抗氧化系统各组分之间存在着共调节,以避免或降低冬季造成的氧化伤害,且灰绿色变异类型的比黄绿色的更能建立较完善的保护系统。
In order to explore the causes of winter leaf color change and its relationship with antioxidation, this paper took the Sabina vulgaris, a yellow-green and gray-green variability type, as the research object, and observed the leaf color and pigment content, the degree of stress and the physiological indexes such as antioxidant system Variety. The results showed that during the natural overwintering process, the yellow-green leaf color changed from yellow-green to orange-red and the gray-green color changed from green to gray-green or gray. At the same time, the yellow-green leaf anthocyanin (Acy) There was no obvious change in the content of chlorophyll, Chl and Car. The content of Chl in the gray-green leaves decreased significantly and the content of Car and Acy increased significantly. H2O2, EL and MDA content increased gradually after winter and reached the highest in January or February of the following year Decreased; the maximum photochemical efficiency (Fv / Fm) showed the opposite trend, the lowest in January next year. In the antioxidant system, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate (As A) and reduced glutathione GSH) showed a similar trend with H2O2, while GSH content in gray-green showed a trend of high-low-high-low. Correlation analysis showed that there was co-regulation between the two types of kudzu pigments and antioxidant systems in order to avoid or reduce the oxidative damage caused by winter, and the types of gray-green variation were more yellow-green To establish a more comprehensive protection system.