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目的:探讨端粒酶抗原冲击的树突状细胞(DCs)在体外诱导抗前列腺癌的免疫效应。方法:细胞因子IL-4和GMCSF诱导前列腺癌患者外周血单核细胞(peripheral blood mononuclear cells,PBMC)生成DCs,将端粒酶逆转录酶(humantelomerase reverse transcriptase,hTERT)mRNA转染DCs使之表达hTERT蛋白,诱导产生肿瘤特异性细胞毒性T细胞(cy-totoxic T lymphocyte,CTL)。MTT法检测负载端粒酶抗原的DCs对T细胞的激发效率,LDH法检测激发产生的CTL对前列腺癌细胞的特异性杀伤作用。结果:端粒酶抗原冲击的DCs较非端粒酶冲击的DCs更能有效诱导T细胞增殖(平均刺激增殖指数分别为10.03±0.88和3.95±0.46,P<0.05),经其诱导产生的T细胞对自体肿瘤细胞的特异性杀伤活性明显高于非端粒酶冲击组[平均肿瘤细胞清除率分别为(62.58±9.44)%和(17.67±3.02)%,P<0.05]。结论:端粒酶抗原冲击的DCs可望成为前列腺癌肿瘤疫苗,在前列腺癌免疫治疗中发挥作用。
Objective: To investigate the immunological effects of dendritic cells (DCs) impacted by telomerase antigen on anti-prostate cancer in vitro. METHODS: DCs were generated from peripheral blood mononuclear cells (PBMCs) of patients with prostate cancer by cytokines IL-4 and GMCSF, and transfected with DCs to express human telomerase reverse transcriptase (hTERT) hTERT protein, induces the production of tumor-specific cytotoxic T lymphocytes (CTLs). The MTT method was used to detect the efficiency of DCs loaded with telomerase antigen on T cells. LDH assay was used to detect the cytotoxic effect of CTLs on prostate cancer cells. Results: DCs impacted by telomerase antigen could induce T cell proliferation more effectively than non-telomerase impacted DCs (average stimulation proliferation index was 10.03 ± 0.88 and 3.95 ± 0.46 respectively, P <0.05). The T The specific cytotoxic activity of the cells against autologous tumor cells was significantly higher than that of the non-telomerase shock groups (mean tumor clearance rate was (62.58 ± 9.44)% vs (17.67 ± 3.02)%, P <0.05). Conclusion: DCs impacted by telomerase antigen are expected to become prostate cancer vaccine and play a role in immunotherapy of prostate cancer.