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目的在mRNA水平上探讨性激素孕酮(Prog)对小鼠脾脏CD11c阳性树突状细胞(SDCs)合成的细胞因子IL-6I、L-10I、L-12和TNF-α的影响。方法采用免疫磁珠法分选小鼠CD11c阳性SDCs,用不同浓度的Prog处理SDCs 24h,采用RT-PCR在mRNA水平上检测SDCs合成细胞因子的变化。结果中浓度的Prog上调IL-6和IL-10的表达而高浓度则降低表达;各浓度组的Prog可上调细胞因子IL-12的水平而降低TNF-α的表达。结论Prog可能通过影响SDCs合成的细胞因子进而调控机体的免疫反应,低、中浓度的Prog使机体的免疫应答向Th2倾斜,而高浓度的Prog推动机体内的免疫反应向Th1型转变。
Objective To investigate the effects of progesterone (Prog) on cytokines IL-6I, L-10I, L-12 and TNF-α synthesized by CD11c positive dendritic cells (SDCs) in mouse spleen at mRNA level. Methods CD11c positive SDCs were sorted by immunomagnetic beads method. SDCs were treated with different concentrations of Prog for 24 hours. The changes of cytokine synthesis in SDCs were detected by RT-PCR. Results Prog increased the expression of IL-6 and IL-10 and decreased the expression of Prog at high concentration. Prog increased the level of IL-12 and decreased the expression of TNF-α. CONCLUSIONS: Prog may regulate the immune response by affecting the synthesis of SDCs. Low and medium concentrations of Prog may cause the body’s immune response to tilt towards Th2, whereas Prog may promote the body’s immune response to Th1-type changes.