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目的:分析急性髓系白血病(AML)细胞上补体调节蛋白CD55、CD59的表达。方法:采用流式细胞仪分析203例初诊的AML白血病细胞膜上的CD55、CD59表达率。结果:203例AML患者中,83例患者白血病细胞CD59表达率下降(占45%),5例患者白血病细胞CD55表达率下降(其中4例为CD55、CD59表达率同时下降),所有患者红系细胞CD55、CD59表达均正常。按照WHO分型诊断标准对患者分类分析,各类型中CD59表达率下降的患者所占比例分别为:AML-M2(16.7%)、AML伴t(8;21)(44.0%)、AML伴t(15;17)(67.92%)、AML-M4(9.52%)、AML伴t(16;16)或inv(16)(92.31%)、AML-M5(27.14%)。4例CD55、CD59表达率同时下降的患者分别为3例AML伴t(15;17),1例AML-M5,单纯CD55表达下降的为1例AML-M4。把预后良好组[AML伴t(8;21)、AML伴t(15;17)、AML伴t(16;16)或inv(16)]的CD59表达率下降患者的比例分别与其他患者(包括AML-M2、AML-M4、AML-M5)下降的比例加以比较,各组均差异有统计学意义(均P<0.01)。结论:AML白血病细胞可以出现CD55、CD59表达率的下降,主要表现为CD59的表达率下降。染色体预后良好的AML细胞上的CD59下降比例,明显高于其他类型AML。
Objective: To analyze the expression of complement regulatory proteins CD55 and CD59 on acute myeloid leukemia (AML) cells. Methods: Flow cytometry was used to analyze the expression of CD55 and CD59 on membrane of 203 newly diagnosed AML leukemia cells. Results: Among 203 AML patients, the expression of CD59 in leukemia cells was decreased in 83 patients (45%), and the expression of CD55 in leukemia cells was decreased in 5 patients (4 of them were CD55 and the expression of CD59 was decreased). All patients with erythroid Cell CD55, CD59 expression were normal. According to the classification criteria of WHO, the proportion of patients with decreased expression of CD59 in each type was AML-M2 (16.7%), AML with t (8; 21) (44.0%), AML with t AML-M4 (9.52%), AML with t (16; 16) or inv (16) (92.31%) and AML-M5 (27.14%). Three patients with AML with t (15; 17) and one with AML-M5, and one patient with a decreased expression of CD55, one patient with AML-M4, had a decreased expression of CD55 and CD59 at the same time. The percentage of patients with a good prognosis group [AML with t (8; 21), AML with t (15; 17), AML with t (16; 16) or inv (16) Including AML-M2, AML-M4, AML-M5), the differences between the two groups were statistically significant (all P <0.01). Conclusion: The expression of CD55 and CD59 in AML leukemia cells may be decreased, which is mainly due to the decreased expression of CD59. The percentage of CD59 decrease in AML cells with good chromosomal prognosis was significantly higher than that of other AML types.