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为探讨姜黄素对甲醛致A549细胞DNA-蛋白质交联(DPC)的拮抗效应,采用培养A549细胞株作为实验材料,分为正常对照组、0.1 mmol/L甲醛组、姜黄素拮抗组(2.5、5.0、10.0、20.0 mg/L姜黄素+0.1 mmol/L甲醛组),对细胞染毒4 h后采用氯化钾-十二烷基硫酸钠沉淀法检测DPC率。结果显示,0.1 mmol/L甲醛染毒组DPC率高于对照组(P<0.05),各姜黄素拮抗组DPC率均低于0.1 mmol/L甲醛组,但2.5、5.0、10.0 mg/L姜黄素拮抗组的DPC率高于对照组,差异均有统计学意义(P<0.05),20.0 mg/L姜黄素拮抗组DPC率与对照组差异无统计学意义(P>0.05)。提示较高浓度的姜黄素可以拮抗甲醛所致的DNA-蛋白质交联损伤。
To investigate the antagonistic effect of curcumin on formaldehyde-induced DNA-protein cross-linking (DPC) in A549 cells, A549 cell line was used as experimental material and divided into normal control group, 0.1 mmol / L formaldehyde group, curcumin antagonist group 5.0, 10.0, 20.0 mg / L curcumin + 0.1 mmol / L formaldehyde). The cells were exposed to potassium chloride-sodium dodecyl sulfate precipitation for 4 h. The results showed that the DPC rate of 0.1 mmol / L formaldehyde exposure group was higher than that of the control group (P <0.05), and the DPC rates of all the curcumin antagonistic groups were lower than that of the 0.1 mmol / L formaldehyde group, but 2.5, 5.0 and 10.0 mg / L turmeric DPC rate in the antagonistic group was higher than that in the control group (P <0.05). There was no significant difference in the DPC rate between the 20.0 mg / L curcumin antagonistic group and the control group (P> 0.05). It suggested that higher concentration of curcumin could antagonize formaldehyde-induced DNA-protein cross-linking injury.