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利用荧光探针JC 1观察大鼠心肌细胞凋亡早期线粒体膜电位的变化。用过氧化氢 (H2 O2 )诱导心肌细胞凋亡 ,在凋亡早期 ,利用新型荧光探针JC 1标记细胞线粒体 ,在FACSCalibur流式细胞仪上检测JC 1荧光强度的变化 ,用Annexin V/PI双染色法鉴别凋亡和坏死的心肌细胞。结果显示 ,在正常大鼠心肌细胞中 ,线粒体维持较高的膜电位 ,JC 1在线粒体内形成聚合物 ,发出红色荧光。H2 O2 导致线粒体膜电位下降 ,JC 1聚合物分解成单体 ,红色荧光强度减弱。提示JC 1结合流式细胞仪是一种理想的检测线粒体膜电位的手段。
Fluorescence probe JC 1 was used to observe the change of mitochondrial membrane potential in rat cardiomyocytes during early apoptosis. The apoptosis of cardiomyocytes was induced by hydrogen peroxide (H2 O2). At the early stage of apoptosis, the changes of JC 1 fluorescence intensity were detected by FACSSCalibur flow cytometry using a new fluorescent probe JC 1, mitochondria were detected by Annexin V / PI Double staining to identify apoptotic and necrotic cardiomyocytes. The results showed that mitochondria maintain a high membrane potential in normal rat cardiomyocytes, and JC 1 forms a polymer in the mitochondria that fluoresces red. H 2 O 2 led to the decrease of mitochondrial membrane potential, the degradation of JC 1 polymer into monomers and the decrease of red fluorescence intensity. Tip JC 1 combined with flow cytometry is an ideal means of detecting mitochondrial membrane potential.