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目的:探讨锌对酒精性肝损害小鼠是否具有保护作用。方法:将50只小鼠随机分为5组,每组各10只,分别为空白对照组、白酒对照组和葡萄糖酸锌低、中、高剂量组。其中,空白对照组和白酒对照组饮用自来水;其他各组分别饮用自由溶于水的不同剂量的葡萄糖酸锌溶液。第30天时对小鼠进行空腹白酒灌胃实验,然后处死小鼠,测定肝匀浆中谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)含量及血谷草转氨酶(AST)、谷丙转氨酶(ALT)。结果:与空白对照组相比,白酒对照组AST、ALT显著升高(P<0.05或P<0.01),MDA显著性升高、GDH-Px活力显著下降(P<0.01)。与白酒对照组相比,高剂量锌组使ALT显著下降(P<0.05),中、高剂量锌组使AST显著下降(P<0.05)。各剂量锌组均可使MDA下降,差异有统计学意义(P<0.05或P<0.01)。结论:白酒可造成肝组织酶异常,对于急性酒精性肝损害小鼠,葡萄糖酸锌均可对肝脏起到保护作用。
Objective: To investigate whether zinc has a protective effect on alcoholic liver injury in mice. Methods: Fifty mice were randomly divided into five groups with 10 rats in each group. They were blank control group, Liquorical control group and low, medium and high doses of zinc gluconate. Among them, the control group and the white wine drinking water control group; other groups were drinking free water soluble different doses of zinc gluconate solution. On the 30th day, the mice were fed with fasting alcohol, and the mice were sacrificed to determine the content of glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and the content of AST in the liver homogenate , Alanine aminotransferase (ALT). Results: Compared with the blank control group, the levels of AST and ALT in liquor control group were significantly increased (P <0.05 or P <0.01), MDA significantly increased, and GDH-Px activity was significantly decreased (P <0.01). Compared with the control group, the high dose zinc group decreased the ALT significantly (P <0.05), and the middle and high dose zinc group decreased the AST significantly (P <0.05). Each dose of zinc can make MDA decreased, the difference was statistically significant (P <0.05 or P <0.01). Conclusion: Liquor can cause abnormal liver tissue enzymes, and zinc gluconate can play a protective role on the liver in acute alcoholic liver damage mice.