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目的 :探讨T淋巴细胞活化及释放的Th1/Th2 细胞因子失平衡与变态反应性鼻炎发病的关系。方法 :用双抗体夹心酶联免疫吸附试验 (ELISA)测定 40例变态反应性鼻炎患者 ,2 0例慢性鼻炎患者和 2 0例健康人的外周血单个核细胞 (PBMC) ,经PHA诱导培养后上清液中Th1细胞因子γ干扰素 (IFN -γ)和Th2 细胞因子白介素 4(IL -4)、白介素 5 (IL -5 )的水平及血清中可溶性白介素 2受体 (sIL -2R)的水平 ,并分别对变应性鼻炎患者的sIL -2R与IFN -γ、IL-4、IL -5的相关性进行研究。结果 :PBMC培养的上清液中变应性鼻炎组的IL -4和IL-5水平显著高于慢性鼻炎组和正常组 (P <0 0 1 ) ;而IFN -γ水平却显著低于后两组(P <0 .0 1 )。变应性鼻炎组患者血清的sIL-2R变应性鼻炎组均显著高于后两组 (P <0 .0 1 )且与PBMC培养的上清液中IL -4和IL -5的浓度呈正相关 (r分别为 0 .62 5和 0 .5 95 ,P <0 .0 1 )。与IFN -γ浓度呈负相关(r=-0 .5 80 ,P <0 .0 1 )。结论 :变应性鼻炎患者体内激活的T淋巴细胞是Th2 细胞 ,并释放Th2 细胞因子 ,Th1细胞和Th1细胞因子分泌则受到抑制 ;Th2 细胞因子在调节变应性鼻炎IgE合成及嗜酸性细胞浸润等病理生理机制上起重要作用
Objective: To investigate the relationship between the imbalance of Th1 / Th2 cytokines and the pathogenesis of allergic rhinitis induced by T lymphocyte activation and release. Methods: Peripheral blood mononuclear cells (PBMCs) from 40 allergic rhinitis patients, 20 chronic rhinitis patients and 20 healthy controls were detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). After induced by PHA The levels of Th1 cytokine interferon (IFN-γ) and Th2 cytokines IL-4 and IL-5 in supernatants and serum levels of soluble interleukin 2 receptor (sIL-2R) Level, and the correlation between sIL -2R and IFN-γ, IL-4, IL-5 in patients with allergic rhinitis were studied. Results: The levels of IL-4 and IL-5 in allergic rhinitis group were significantly higher than those in chronic rhinitis group and normal group (P <0.01), while the levels of IFN-γ in PBMC culture supernatant were significantly lower than those in the control group Two groups (P <0. 01). Serum levels of sIL-2R allergic rhinitis in patients with allergic rhinitis were significantly higher than those in the latter two groups (P <0.01), and the concentrations of IL-4 and IL-5 in supernatants from PBMCs were positive (R = 0.62 5 and 0.55, respectively, P <0.01). And IFN-γ concentration was negatively correlated (r = -0.580, P <0.01). Conclusion: The activation of T lymphocytes in patients with allergic rhinitis is Th2 cells and release of Th2 cytokines, Th1 cells and Th1 cytokine secretion is inhibited; Th2 cytokines in the regulation of allergic rhinitis IgE synthesis and eosinophil infiltration Other pathophysiological mechanisms play an important role