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目的 研究培养兔角膜内皮细胞,为实验提供基础。方法 通过改良的细胞收获、培养技术,在体外进行兔角膜内细胞的培养,细胞的类型由电镜证实。观察hEGF不同时间点对角膜内皮细胞生长状态的影响。结果 未加入任何促细胞裂剂的情况下,细胞在体外生长良好,约一周左右形成密集状态,形态类似天然细胞,经倒置显微镜和电镜证实为角膜内细胞。在有hEGF存在时,细胞生长迅速,3-4天形成单层,各个时间点EGF组的细胞数高于对照组。结论 该技术可为角内皮细胞的研究提供较多的纯内皮细胞。
Objective To study the culture of rabbit corneal endothelial cells, providing the basis for the experiment. Methods The cultured cells in rabbit corneas were cultured in vitro by modified techniques of cell harvesting and culture. The types of cells were confirmed by electron microscopy. The effects of hEGF at different time points on the growth of corneal endothelial cells were observed. Results In the absence of any mitogen, the cells grew well in vitro and formed a dense state in about one week. The morphology of the cells was similar to that of natural cells. Cells were confirmed by inverted microscope and electron microscope. In the presence of hEGF, the cells grew rapidly, forming a monolayer in 3-4 days, and the number of cells in the EGF group at each time point was higher than that in the control group. Conclusion This technique can provide more pure endothelial cells for the study of corneal endothelial cells.