论文部分内容阅读
目的探讨夏枯草对二甲基苯并蒽(DMBA)诱发的金黄地鼠口腔癌的化学预防作用。方法2006年3月在首都医科大学口腔医学院研究所将30只金黄地鼠分3组:阴性对照组(6只)不涂药;阳性对照组(12只)涂0.5%DMBA于左侧颊囊,每周3次,共涂3周;夏枯草组(12只)前3周处理同阳性对照组,3周后换涂1%夏枯草,每周3次,涂1周。第4周末实验结束处死所有地鼠,处死前2h腹腔注射50mg/kg的5-溴脱氧尿嘧啶(5-bromo-2’-deoxyuridine,BrdU)。取左侧颊囊行组织病理学观察和BrdU免疫组化染色。结果与阳性对照组相比,夏枯草组颊囊单纯增生和异常增生的病灶数目均有所降低,其单纯增生病灶数目与阳性对照组比较差异有统计学意义(P<0.05)。夏枯草组炎症细胞数(52.25±18.53)个/mm2与阳性对照组(158.65±26.51)个/mm2相比,差异有统计学意义(P<0.01)。夏枯草组BrdU阳性率与阳性对照组相比有所降低,但二者差异无统计学意义。结论夏枯草对DMBA诱发的金黄地鼠的炎症和口腔癌前病变的单纯增生有一定的抑制作用,但对于异常增生和细胞增殖抑制作用不明显。
Objective To investigate the chemopreventive effects of Prunella vulgaris on DMBA-induced oral halotus oral carcinoma. Methods Thirty golden hamsters were divided into three groups at the Institute of Stomatology, Capital Medical University in March 2006: negative control group (6) without drug application; positive control group (12) with 0.5% DMBA on left cheek 3 weeks, a total of 3 weeks; Prunella group (12) 3 weeks before treatment with the same positive control group, 3 weeks after the exchange of 1% Prunella, 3 times a week for 1 week. At the end of the fourth week, all the rats were sacrificed at the end of the experiment and 5 mg / kg of 5-bromo-2’-deoxyuridine (BrdU) was intraperitoneally injected 2 h before the sacrifice. Left cheek pouch line histopathological observation and BrdU immunohistochemical staining. Results Compared with the positive control group, the number of hyperplastic prion hyperplasia and hyperplastic prostatic hyperplasia in the Prunella vulgaris group was decreased, and the number of hyperplastic lesions was significantly different from that in the positive control group (P <0.05). The number of inflammatory cells in Prunella vulgaris group (52.25 ± 18.53) / mm2 was significantly higher than that in positive control group (158.65 ± 26.51) / mm2 (P <0.01). Prunella group BrdU positive rate compared with the positive control group decreased, but the difference was not statistically significant. Conclusion Prunella vulgaris has some inhibitory effects on the inflammation induced by DMBA and the simple hyperplasia of oral precancerous lesion, but has no obvious inhibitory effect on dysplasia and cell proliferation.