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向小鼠采用一次及多次注入Ｔ细胞促有丝分裂剂植物血凝素铝吸附物（Ａｌｕｍ－ＰＨＡ）后，应用免疫组化方法观察淋巴小结的形成。实验分 １次注入及 ３次注入组，不同时期取出 淋巴结后，用免疫组化法、三维重建进行观察。结果：（１）淋巴结中大量次级淋巴小结形成，说明淋巴小结的形成不但与刺激Ｔ细胞有关，而且与活化巨噬细胞有关。（２）１／３量注入组产生的淋巴小结数比全量１次注入组产生的多；但第１２周末不同组别产生的淋巴小结数差别不大，１２周末消退了许多生发中心。可见反应性淋巴小结的形成，不仅与刺激物性状和投入量有关，而且与投入方式有关。
The mice were used to inject T cell mitogen phytohemagglutinin aluminum adsorbent (Alum-PHA) once or several times, and immunohistochemistry was used to observe the formation of lymphoid nodules. The experiment was divided into 1 injection and 3 injection groups. Lymph nodes were removed at different stages and observed by immunohistochemistry and 3D reconstruction. Results: (1) A large number of lymph nodes in the formation of secondary lymph nodes, lymph node formation not only to stimulate the formation of T cells, but also with activated macrophages. (2) One third of the injected groups produced more lymph nodes than those injected with the same amount of the whole group. However, the number of lymphatic nodules produced by different groups was not significantly different at the 12th week, and many germinal centers subsided at the end of the 12th week. Shows the formation of reactive lymph nodes, not only with stimulus traits and input related, but also with the input method.