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文献报导恶性肿瘤的发生和生长与某些微量金属有密切的关系。鉴于絡合剂可影响机体微量元素的代謝,所以作者在研究Sb-71的作用机制时,着重探討它及其类似物对Zn~(65)渗入瘤細胞的影响,結果如下: (1)Sb-71及其类似物(ATA以及Hg,Bi,Pb,Sn和Ba的EDTA螫合物)以不同浓度加入Ehrlich瘤細胞腹水液,在体外37℃溫孵,在3小时內均对Zn~(65)渗入瘤細胞有一定的抑制作用,其抑制率为22—60％不等。其中以ATA和Sb-71的抑制作用較强。但吐酒石则无明显的抑制作用。 (2)Sb-71剂量30毫克/公斤腹腔注射,对Zn~(65)渗入小鼠Ehrlich腹水瘤細胞有非常明显的抑制作用,以給药后5—7小时作用最显著,主要是使核蛋白中的脉冲数減少,12小时后逐渐接近于对照組水平。渗入肝組織蛋白的放射强度并不受药物的影响,表示其作用有明显的組織选择性。ATA同样剂量腹腔注射則无明显的抑制效能,相反的可以促进腹水清液中Zn~(65)的減少。 (3)EDTA-Hg、EDTA-Bi和EDTA-Pb剂量分别为4,10和200毫克/公斤腹腔注射时,对Zn~(65)渗入小鼠Ehrlich腹水瘤細胞的影响均不如Sb-71明显,除EDTA-Hg組总氮脉冲数較对照組明显減少外,其他与对照組比較均无显著的差异。
Literature reports the occurrence and growth of malignant tumors and some trace metals are closely related. As the complexing agent can affect the metabolism of trace elements in the body, the author focuses on the mechanism of Sb-71 when it is used to investigate the effect of complexing agent on Zn ~ (65) infiltration into tumor cells. The results are as follows: (1) Sb- 71 and its analogs (ATA and EDTA complexes of Hg, Bi, Pb, Sn and Ba) were added to Ehrlich tumor cells in different concentrations. After in vitro incubation at 37 ℃, Zn ~ (65 ) Into the tumor cells have a certain inhibitory effect, the inhibition rate of 22-60% range. Among them, the inhibition of ATA and Sb-71 is stronger. However, spit tartar no significant inhibitory effect. (2) The intraperitoneal injection of Sb-71 at a dose of 30 mg / kg significantly inhibited the infiltration of Zn ~ (65) into mouse Ehrlich ascites tumor cells, and the most obvious effect was 5-7 hours after administration, The number of pulses in the protein decreased, approaching the control level gradually after 12 hours. Infiltration of liver tissue protein radiation intensity is not affected by the drug, indicating that its role in a significant tissue selectivity. The same dose of ATA intraperitoneal injection had no significant inhibitory effect, on the contrary can promote the reduction of Zn ~ (65) in ascites fluid. (3) The effect of Zn ~ (65) infiltration into mouse Ehrlich ascites tumor cells was not as obvious as that of Sb-71 when the doses of EDTA-Hg, EDTA-Bi and EDTA-Pb were 4, 10 and 200 mg / , Except that the total nitrogen pulse number in EDTA-Hg group was significantly decreased compared with the control group, and the other groups had no significant difference compared with the control group.