Southern吸印法是进行组份分析及研究其表达的基本技术之一。近年来,由于采用了尼龙膜,从而明显增加了这一技术在杂交分析中的应用。与硝酸纤维膜相比,尼龙膜在核酸杂交中具有以下几个优点:①机械强度较高;②提高了核酸的载量;③能够结合更小的寡核苷酸片段;④提高了与核酸结合的能力,因而可以多次重复杂交;⑤该膜能提 Southern blotting is one of the basic techniques for analyzing components and studying their expression. In recent years, due to the use of nylon membrane, which significantly increased the application of this technology in hybridization analysis. Compared with the nitrocellulose membrane, nylon membrane in nucleic acid hybridization has the following advantages: ① higher mechanical strength; ② increased nucleic acid load; ③ can be combined with smaller oligonucleotide fragments; ④ increased with the nucleic acid Combined with the ability to repeat the hybridization can be repeated; ⑤ the membrane can mention