论文部分内容阅读
以唐古特白刺为试材,在单因素试验的基础上,选取液料比、超声时间、超声功率和提取温度为影响因子,应用Box-Behnken中心组合设计建立数学模型,以蛋白质得率为响应值,进行响应面分析。结果表明:超声波提取唐古特白刺中蛋白质的最佳工艺条件为超声时间30min,超声温度50℃,液料比为20∶1mL/g,超声功率150W,提取次数2次,在此条件下唐古特白刺蛋白质得率达到(11.87±0.32)%。响应面法建立的数学模型和试验数据相符,优化的唐古特白刺中蛋白质提取工艺方法简便,为进一步开发唐古特白刺蛋白提供了依据。
Based on the single factor experiments, the liquid to material ratio, ultrasonic time, ultrasonic power and extraction temperature were selected as the influencing factors. The mathematical model was set up using the Box-Behnken central combination design. The yield of protein was Response values, response surface analysis. The results showed that the optimum extraction conditions for protein in the Tangut as a result of ultrasonication were as follows: ultrasonic time 30min, ultrasonic temperature 50 ℃, liquid-to-liquid ratio 20:1mL / g, ultrasonic power 150W, extraction times 2 times, Extra thorns protein yield reached (11.87 ± 0.32)%. The mathematical model established by response surface method is in good agreement with the experimental data. The optimized protein extraction method of Nitraria tangutorum is simple and convenient, which provides the basis for further development of Nitraria tangutorum protein.