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用 Giemsa 染色观察了细脚拟青霉(Paecilomyces tenuipes)803和1401菌株间的菌丝联结现象,观察了菌丝、孢子的细胞核数目。孢子均是单核,菌丝细胞有1—2个核,多数为单核,菌丝尖端有1—6个核。毒力测定结果表明:能形成异核体的亲和单孢子菌株组合803-17和1401-9、803-3和1401-8的混合培养物毒力与未经培养的相同菌株组合的菌丝体混合物毒力显著不同。在讨论申提出这种差异的原因是:在 Sm 液体培养中,混合接种803-17和1401-9、803-3和1410-8,经11天的混合培养后,混合培养物中形成了与核体亲本菌丝(803-17、1401-9、803-3、1401-8)毒力显著不同的异核菌丝体(803-17×1401-9)、(803-3×1401-8)。
The mycelial connections between 803 and 1401 strains of Paecilomyces tenuipes were observed by Giemsa staining. The number of nuclei in mycelium and spores was observed. Spores are mononuclear, mycelial cells have 1-2 nuclei, most of the mononuclear, hyphal tip 1-6 nuclei. Toxicity assay results show that the hyphae of virulent mixed monophosphates capable of forming heterokaryon combinations 803-17 and 1401-9, 803-3 and 1401-8, in combination with the same strain without the culture The bulk mixture is significantly different in virulence. The reason for this discrepancy in the discussion is that in Sm liquid culture, 803-17 and 1401-9, 803-3 and 1410-8 were mixed inoculated and mixed with Nucleocapsid mycelium (803-17,1401-9,803-3,1401-8) virulence significantly different heterokaryon mycelium (803-17 × 1401-9), (803-3 × 1401-8 ).